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1G!gƒ¼%g|‘k#pÁÌä+  fgñò­÷Ù@	056¢ãïø7@{�±Z„Ý"mno¿ÀdefšÇàá  .0  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                     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BASE      F,  xÁA  ƒ°YëñÀ þµ,9K�lMæ$"¥�æÜ€‘ÔH©…Ûjk8b´¦Î›¤4Ù¤¢9·ÂµÐÚ€p4ÆfÅÜ–“*‰�¶YmÉÅ„pdBÑ,aÂ"ËJ€ 0¡ �nš0F´µm9ÝV²�•‘³iÆŠV¤ª©¦ÊtL6U§+uslM¦,Iu‰¦-¥È)ºÍ&©MÕÀ�l¢ÈjQÔLÓ"s �QÂD ‰€RQ@AEÃBP* c“h[¨J@5µÀU3©†2°måjj.-†ÍTXäšC«B rX:ƒaQmÍtDY°�tccÄ´�VT¹jmêªW¡ÐJkÛ¶œäª�m[µ!nµ™n™³¦fºÍ ¦0™²m¶d›,‰b«“¦™n€I sê6W²IZP�œ,m  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>  s     ^®ZTR

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
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:51:12">2019.6.4</Created>
<LastModified UTC="9:27:42">2022.1.20</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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h  ^      I®ZTR

BASE      3è  xÁA  ƒ°YëñÀ þµ,9–éŒÕdN"RÚhÎI�”Z¸­¶†#Fkê°IJ“M*šs+\] ç@Û¬¶�äbB82¡h–0a‘e%@ ˜PPH7M#ƒ
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
BASE      2è  xÁA  ƒ°YëñÀ þµ,9V¦[“9‰Hi£97`$5Rjá¶ÚŽ­©sÀ&)M6©hÎ­p-t�6 œ�±D1·å¤Jb mVÛ@r1!™P4K˜°È²  L((¤›&Œ€‘Amm[N·•,deälš±¢©jª©2“MÕéJÝ[“)KR]¢©DK)rŠnsƒIªDSµ0D ›(²ÚD5Ó´†ÈÄHc”0h" A…TPPÂ°”
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
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
BASE      H2  xÁA  ƒ°YëñÀ þµ,9ÆÒdk2')�æÜ€‘ÔH©…Ûjk8b´¦Î›¤4Ù¤¢9·ÂµÐÚ€p4'Û&±ŠÜœŽ2Úfµ$Â‘	E³„	‹,+À„‚r@ºiÂTÐÖ¶åt[ÉBVFÎ¦+Z‘ª¦š*Ó1ÙT�®ÔÍ±5™²$Õ%šJ´”"§è67˜¤J4U[ C²‰"«MDQ3MkˆLÌ�4F	�&THE!A© ŒM¢m¡*ÕÔWÍ¤Ê À¶m”«©¹´6Sa!�k­
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
BASE      LD  xÁA  ƒ°YëñÀ þµ,¹²m­5™“ˆ”6šsFR#¥n«­áˆÑš:l’Òd“ŠæÜ
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  d<Notes>
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:51:12">2019.6.4</Created>
<LastModified UTC="9:27:4">2022.1.20</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<LastModified UTC="5:31:26">2021.6.17</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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  e<Notes>
<UUID>125c378e-db42-49c9-8328-7b1d032919b3</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:51:12">2019.6.4</Created>
<LastModified UTC="11:54:3">2019.6.13</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<SequenceClass>UNA</SequenceClass>
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arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="2" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3409-3994" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4956" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4165-4956" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="4957-5025" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  ã<?xml version="1.0"?><Primers recycledIDs="21,19,1,4,14,18,7,13,20,6" nextValidID="23"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="8" name="Primer 1" sequence="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2077-2326" boundStrand="0" annealedBases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag" meltingTemperature="91"><Component hybridizedRange="2077-2326" bases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag"/></BindingSite><BindingSite simplified="1" location="2077-2326" boundStrand="0" annealedBases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag" meltingTemperature="91"><Component hybridizedRange="2077-2326" bases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag"/></BindingSite></Primer><Primer recentID="22" name="M13R" sequence="CAGGAAACAGCTATGAC"><BindingSite location="3006-3022" boundStrand="1" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="3006-3022" bases="CAGGAAACAGCTATGAC"/></BindingSite><BindingSite simplified="1" location="3006-3022" boundStrand="1" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="3006-3022" bases="CAGGAAACAGCTATGAC"/></BindingSite></Primer><Primer recentID="17" name="V1_5" sequence="GGACTTTCCAAAATGTCG"><BindingSite location="730-747" boundStrand="0" annealedBases="GGACTTTCCAAAATGTCG" meltingTemperature="51"><Component hybridizedRange="730-747" bases="GGACTTTCCAAAATGTCG"/></BindingSite><BindingSite simplified="1" location="730-747" boundStrand="0" annealedBases="GGACTTTCCAAAATGTCG" meltingTemperature="51"><Component hybridizedRange="730-747" bases="GGACTTTCCAAAATGTCG"/></BindingSite></Primer><Primer recentID="16" name="HH-pBRori-cexuR" sequence="TCGGGTTTCGCCACCTCTG"><BindingSite location="3460-3478" boundStrand="1" annealedBases="TCGGGTTTCGCCACCTCTG" meltingTemperature="61"><Component hybridizedRange="3460-3478" bases="TCGGGTTTCGCCACCTCTG"/></BindingSite><BindingSite simplified="1" location="3460-3478" boundStrand="1" annealedBases="TCGGGTTTCGCCACCTCTG" meltingTemperature="61"><Component hybridizedRange="3460-3478" bases="TCGGGTTTCGCCACCTCTG"/></BindingSite></Primer><Primer recentID="15" name="HH-L-cexuF" sequence="ACGACAGGTTTCCCGACTG"><BindingSite location="3148-3166" boundStrand="1" annealedBases="ACGACAGGTTTCCCGACTG" meltingTemperature="59"><Component hybridizedRange="3148-3166" bases="ACGACAGGTTTCCCGACTG"/></BindingSite><BindingSite simplified="1" location="3148-3166" boundStrand="1" annealedBases="ACGACAGGTTTCCCGACTG" meltingTemperature="59"><Component hybridizedRange="3148-3166" bases="ACGACAGGTTTCCCGACTG"/></BindingSite></Primer><Primer recentID="12" name="PQE-F" sequence="TGAGCGGATAACAATTTCAC"><BindingSite location="3024-3043" boundStrand="1" annealedBases="TGAGCGGATAACAATTTCAC" meltingTemperature="52"><Component hybridizedRange="3024-3043" bases="TGAGCGGATAACAATTTCAC"/></BindingSite><BindingSite simplified="1" location="3024-3043" boundStrand="1" annealedBases="TGAGCGGATAACAATTTCAC" meltingTemperature="52"><Component hybridizedRange="3024-3043" bases="TGAGCGGATAACAATTTCAC"/></BindingSite></Primer><Primer recentID="11" name="PCMV-R" sequence="TCCAAACTCATCAATGTATC"><BindingSite location="2935-2954" boundStrand="0" annealedBases="TCCAAACTCATCAATGTATC" meltingTemperature="49"><Component hybridizedRange="2935-2954" bases="TCCAAACTCATCAATGTATC"/></BindingSite><BindingSite simplified="1" location="2935-2954" boundStrand="0" annealedBases="TCCAAACTCATCAATGTATC" meltingTemperature="49"><Component hybridizedRange="2935-2954" bases="TCCAAACTCATCAATGTATC"/></BindingSite></Primer><Primer recentID="10" name="PIRES-R" sequence="CACTGCATTCTAGTTGTGGT"><BindingSite location="2912-2931" boundStrand="0" annealedBases="CACTGCATTCTAGTTGTGGT" meltingTemperature="56"><Component hybridizedRange="2912-2931" bases="CACTGCATTCTAGTTGTGGT"/></BindingSite><BindingSite simplified="1" location="2912-2931" boundStrand="0" annealedBases="CACTGCATTCTAGTTGTGGT" meltingTemperature="56"><Component hybridizedRange="2912-2931" bases="CACTGCATTCTAGTTGTGGT"/></BindingSite></Primer><Primer recentID="9" name="SV40pA-R" sequence="GAAATTTGTGATGCTATTGC"><BindingSite location="2873-2892" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="51"><Component hybridizedRange="2873-2892" bases="GAAATTTGTGATGCTATTGC"/></BindingSite><BindingSite simplified="1" location="2873-2892" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="51"><Component hybridizedRange="2873-2892" bases="GAAATTTGTGATGCTATTGC"/></BindingSite></Primer><Primer recentID="5" name="EBV-Reverse" sequence="CAGCTTATAATGGTTACAAAT"><BindingSite location="2849-2869" boundStrand="0" annealedBases="CAGCTTATAATGGTTACAAAT" meltingTemperature="48"><Component hybridizedRange="2849-2869" bases="CAGCTTATAATGGTTACAAAT"/></BindingSite><BindingSite simplified="1" location="2849-2869" boundStrand="0" annealedBases="CAGCTTATAATGGTTACAAAT" meltingTemperature="48"><Component hybridizedRange="2849-2869" bases="CAGCTTATAATGGTTACAAAT"/></BindingSite></Primer><Primer recentID="3" name="CMV-Forward" sequence="CGCAAATGGGCGGTAGGCGTG"><BindingSite location="768-788" boundStrand="0" annealedBases="CGCAAATGGGCGGTAGGCGTG" meltingTemperature="65"><Component hybridizedRange="768-788" bases="CGCAAATGGGCGGTAGGCGTG"/></BindingSite><BindingSite simplified="1" location="768-788" boundStrand="0" annealedBases="CGCAAATGGGCGGTAGGCGTG" meltingTemperature="65"><Component hybridizedRange="768-788" bases="CGCAAATGGGCGGTAGGCGTG"/></BindingSite></Primer><Primer recentID="2" name="PEGFP-N-5" sequence="TGGGAGGTCTATATAAGCAGAG"><BindingSite location="794-815" boundStrand="0" annealedBases="TGGGAGGTCTATATAAGCAGAG" meltingTemperature="54"><Component hybridizedRange="794-815" bases="TGGGAGGTCTATATAAGCAGAG"/></BindingSite><BindingSite simplified="1" location="794-815" boundStrand="0" annealedBases="TGGGAGGTCTATATAAGCAGAG" meltingTemperature="54"><Component hybridizedRange="794-815" bases="TGGGAGGTCTATATAAGCAGAG"/></BindingSite></Primer><Primer recentID="0" name="M13R(-48)" sequence="AGCGGATAACAATTTCACACAGGA"><BindingSite location="3018-3041" boundStrand="1" annealedBases="AGCGGATAACAATTTCACACAGGA" meltingTemperature="58"><Component hybridizedRange="3018-3041" bases="AGCGGATAACAATTTCACACAGGA"/></BindingSite><BindingSite simplified="1" location="3018-3041" boundStrand="1" annealedBases="AGCGGATAACAATTTCACACAGGA" meltingTemperature="58"><Component hybridizedRange="3018-3041" bases="AGCGGATAACAATTTCACACAGGA"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>bef72f78-a4ea-4aa3-b79c-32d55cec59e2</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.29</Created>
<LastModified UTC="14:0:49">2019.5.5</LastModified>
<CreatedBy>æµ·å�‰æµ©æ ¼</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ¹<StrandColors><TopStrand><ColorRange range="2077..2352" colors="light blue"/></TopStrand><BottomStrand><ColorRange range="2077..2352" colors="light blue"/></BottomStrand></StrandColors>  B«       )         )¿þ`�îÒu¸îód¥=&ã¿ŽˆŠ,á$ŒÄÕjS�uC¡ñÖ�…ã¤
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  ö<?xml version="1.0"?><Features nextValidID="16"><Feature recentID="15" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3031-3047" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3007-3023" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3100-3121" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3055-3061" color="#ffffff" type="standard"/><Segment range="3062-3079" color="#ffffff" type="standard"/><Segment name="-35" range="3080-3085" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5026-5130" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2837-2958" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1920-2055" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1028-1252" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1740-2069" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1298-1726" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="2" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3409-3994" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4956" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4165-4956" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="4957-5025" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2078-2677" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; 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  ÷<?xml version="1.0"?><Primers recycledIDs="21,19,1,4,14,18,7,13,20,6" nextValidID="23"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="8" name="Primer 1" sequence="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2077-2326" boundStrand="0" annealedBases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgaccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag" meltingTemperature="90"><Component hybridizedRange="2077-2127" bases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccc"/><Component bases="a"/><Component hybridizedRange="2129-2189" bases="gggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcga"/><Component bases="t"/><Component hybridizedRange="2191-2326" bases="ccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag"/></BindingSite><BindingSite simplified="1" location="2077-2326" boundStrand="0" annealedBases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgaccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag" meltingTemperature="90"><Component hybridizedRange="2077-2127" bases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccc"/><Component bases="a"/><Component hybridizedRange="2129-2189" bases="gggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcga"/><Component bases="t"/><Component hybridizedRange="2191-2326" bases="ccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag"/></BindingSite></Primer><Primer recentID="22" name="M13R" sequence="CAGGAAACAGCTATGAC"><BindingSite location="3006-3022" boundStrand="1" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="3006-3022" bases="CAGGAAACAGCTATGAC"/></BindingSite><BindingSite simplified="1" location="3006-3022" boundStrand="1" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="3006-3022" bases="CAGGAAACAGCTATGAC"/></BindingSite></Primer><Primer recentID="17" name="V1_5" sequence="GGACTTTCCAAAATGTCG"><BindingSite location="730-747" boundStrand="0" annealedBases="GGACTTTCCAAAATGTCG" meltingTemperature="51"><Component hybridizedRange="730-747" bases="GGACTTTCCAAAATGTCG"/></BindingSite><BindingSite simplified="1" location="730-747" boundStrand="0" annealedBases="GGACTTTCCAAAATGTCG" meltingTemperature="51"><Component hybridizedRange="730-747" bases="GGACTTTCCAAAATGTCG"/></BindingSite></Primer><Primer recentID="16" name="HH-pBRori-cexuR" sequence="TCGGGTTTCGCCACCTCTG"><BindingSite location="3460-3478" boundStrand="1" annealedBases="TCGGGTTTCGCCACCTCTG" meltingTemperature="61"><Component hybridizedRange="3460-3478" bases="TCGGGTTTCGCCACCTCTG"/></BindingSite><BindingSite simplified="1" location="3460-3478" boundStrand="1" annealedBases="TCGGGTTTCGCCACCTCTG" meltingTemperature="61"><Component hybridizedRange="3460-3478" bases="TCGGGTTTCGCCACCTCTG"/></BindingSite></Primer><Primer recentID="15" name="HH-L-cexuF" sequence="ACGACAGGTTTCCCGACTG"><BindingSite location="3148-3166" boundStrand="1" annealedBases="ACGACAGGTTTCCCGACTG" meltingTemperature="59"><Component hybridizedRange="3148-3166" bases="ACGACAGGTTTCCCGACTG"/></BindingSite><BindingSite simplified="1" location="3148-3166" boundStrand="1" annealedBases="ACGACAGGTTTCCCGACTG" meltingTemperature="59"><Component 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  c<Notes>
<UUID>bef72f78-a4ea-4aa3-b79c-32d55cec59e2</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.29</Created>
<LastModified UTC="14:0:38">2019.5.5</LastModified>
<CreatedBy>æµ·å�‰æµ©æ ¼</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<Type>Synthetic</Type>
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<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:49:20">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<LastModified UTC="3:49:10">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<UUID>e6875514-1d5c-48a6-93f8-ffb14f8af1fe</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:44:14">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<LastModified UTC="3:40:28">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:36:6">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<LastModified UTC="3:30:42">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:28:32">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<LastModified UTC="3:26:42">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  Ñ<Notes>
<UUID>d52682b6-dee2-4e13-8c6e-1ef2789f41ba</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-MAP3K7(human)-Myc-SV40-Puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="5:51:12">2019.6.4</Created>
<LastModified UTC="12:15:41">2025.9.15</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y      K         
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   )<?xml version="1.0"?><CustomEnzymeSets/>
